ELISpot Flex: Goat IL-5 (HRP)

ELISpot Flex: Goat IL-5 (HRP)

3193-2H

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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT52B34
Detection mAbMT11B37, biotin
Enzyme conjugateStreptavidin-HRP

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Delivery 4-9 business days

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ELISpot substrate: TMB for HRP


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting goat IL-5 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Goat IL-5 (HRP)
ApplicationELISpot
AnalyteIL-5
ReactivityGoat
SpecificityThis kit is based on a matched pair of mAbs enabling specific detection of goat IL-5.
Cross-reactivityThe mAbs react with bovine IL-5 and cross-react with IL-5 from goat. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.
Goat PBMC (200,000 cells/well) were incubated for 40 h in the absence or presence of PMA/ionomycin and the number of cells secreting IL-5 was analyzed by ELISpot.
Datasheet/Protocol
SDS

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IL-5

Analyte description

Interleukin 5 (IL-5) is a pleiotropic cytokine primarily produced by T and NK cells.  IL-5 specifically controls the survival, differentiation, and chemotaxis of eosinophils. But initially, IL-5 was characterized by its ability to support the growth and terminal differentiation of B cells.

Alternative namesInterleukin-5, IL-5, IL5, BCGF-2, TRF
Cell typeT cell, Th2, NK cell
Gene ID280825